Sondas para Northern-Blot (não reativas)
Produtos
HighYield T7 Digoxigenin RNA Labeling Kit RNT-101-DIGX
Preparation of randomly Digoxigenin-modified RNA probes by in vitro transcription with Dioxigenin-11-UTP
HighYield T7 Biotin16 RNA Labeling Kit RNT-101-BIO16
Preparation of randomly Biotin-modified RNA probes by in vitro transcription with Biotin-16-UTP
Selected References
[1] Miller et al. (2018) Near-infrared fluorescent northern blot. RNA 24(12):1871.
[2] O'Neill JW et al. (1994) Double-label in situ hybridization using biotin and digoxigenin-tagged RNA probes. Biotechniques 17(5):870.
[3] Brauburger et al. (2017) Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA. Methods in Molecular Biology 1628: DOI 10.1007/978-1-4939-7116-9_11.
[4] Melton et al. (1984) Efficient in vitro synthesis of biologicaly active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promotor. Nucleic Acids Research 12(18):7035.
[5] Srivastava et al. (2000) Analysis of RNA by Northern Blotting Using Riboprobes. The Nucleic Acid Protocols Handbook 38:249.
[6] Srivastava et al. (1991) Use of riboprobes for Northern blotting analysis. Biotechniques 11:584.