Marcação Proteica
Table 1: Matriz de Seleção para Marcação Proteica
Type of Label | |||||||
Fluorophore | Biotin | Desthiobiotin | CLICK functionality | ||||
Protein Source | Purified protein | Amine Labeling | Amine Labeling | Amine Labeling | random | Labeling Position | |
Thiol Labeling | Thiol Labeling | ||||||
Proliferating cells (Nascent proteins) | Amino acid-based Metabolic Labeling | ||||||
Puromycin-based Metabolic Labeling | site directed | ||||||
ATP dependent Lysine-based Labeling | ATP dependent Lysine-based Labeling | ||||||
Protein encoding DNA sequence (Cloning & cell-free expression required) | dC-Puromycin-based Co-translational Labeling | dC-Puromycin-based Co-translational Labeling |