TC21/R-Ras2^His (Q72L)

For in vitro use only!

Envio: shipped on blue ice

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Número de acesso: NM_012250

Número de acesso: NM_012250

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 25 mM HEPES pH 7.5, 50 mM NaCl, 5 mM MgCl₂, 1 μM GDP and 50% glycerol)

Descrição:
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. The Ras family of GTP-binding proteins consists of the classical Ras (Ha-, K-, and N-), R-Ras, Rap1, Rap2, Ral, Rheb, Rin, Rit, TC21/R-Ras2, and M-Ras/R-Ras3 (M-Ras). TC21 is a Ras-like GTPase with high oncogenic potential that is found mutated in some human tumors and overexpressed in breast cancer cell lines. It binds physically to c-Raf-1 in a GTP-dependent manner. TC21 (Q72L) is a constitutive active mutant of TC21.

Referências selecionadas:
Movilla et al. (1999) Signal transduction elements of TC21, an oncogenic member of the R-Ras subfamily of GTP-binding proteins. Oncogene. 18:5860.
Huang et al. (2004) Role of TC21/R-Ras2 in enhanced migration of neurofibromin-deficient Schwann cells. Oncogene. 23:368.
Ohba et al. (2000) Regulatory proteins of R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3. J. Biol. Chem. 27:20020.