N-Ras^His

For in vitro use only!

Envio: shipped on blue ice

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 25 kDa

Número de acesso: NM_010937.2

Número de acesso: NM_010937.2

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in PBS pH 7.5, 5 mM MgCl₂ and 50 % glycerol)

Descrição:
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signalling and transport pathways. Protein preparation is 100 % GDP-loaded, measured by HPLC.
N-Ras is a full length protein containing Amino acids 1-189. The 6His-tag is located at the N-terminus.

Referências selecionadas:
Sasazuki et al. (2005) Transformation by Oncogenic RAS Sensitizes
Human Colon Cells to TRAIL-induced Apoptosis by Up-regulating Death Receptor 4 and Death Receptor 5 through a MEK-dependent Pathway. J. Biol. Chem. 280:22856.
Wittinghofer et al. (2000) Ras - a molecular switch involved in tumor formation. Angew. Chem. Int. Ed. 39:4192.
Li et al. (1997) Uncoupling of membrane ruffling and pinocytosis
during Ras signal transduction. J. Biol. Chem. 272:10337.
Pacold et al. (2000) Crystal structure and functional analysis of Ras
binding to its effector Phosphoinositide 3-kinase. Cell 103:931.
Li et al. (2004) Transformation Potential of Ras Isoforms Correlates with Activation of Phosphatidylinositol 3-Kinase but Not ERK. J. Biol. Chem. 279:37398.