H-Ras (Q61L)

For general laboratory use.

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 21.3 kDa (189 amino acids)

Número de acesso: NP_005334

Número de acesso: NP_005334

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 64 mM Tris-HCl pH 7.2, 10 mM MgCl2 and 5 mM DTE)

Descrição:
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and K-Ras4B). The mutation Q61L results in a decreased GTPase activity as well as increased GDP/GTP exchange. This mutant constitutively activates the Ras-signaling pathway. Protein preparation is 77% GDP- and 23% GTP-loaded, measured by HPLC.

Referências selecionadas:
Sasazuki et al. (2005) Transformation by Oncogenic RAS Sensitizes Human Colon Cells to TRAIL-induced Apoptosis by Up-regulating Death Receptor 4 and Death Receptor 5 through a MEK-dependent Pathway. J Biol. Chem. 280:22856. Wittinghofer et al. (2000) Ras - a molecular switch involved in tumor formation. Angew. Chem. Int. Ed. 39:4192. Frech et al. (1994) Role of glutamine-61 in the hydrolysis of GTP by p21H-ras: an experimental and theoretical study. Biochemistry 33:3237.