H-Ras ΔC (S17N)

For general laboratory use.

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 19.5 kDa (171 amino acids)

Número de acesso: NP_005334

Número de acesso: NP_005334

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 64 mM Tris-HCl pH 7.2, 10 mM MgCl2 and 5 mM DTE)

Descrição:
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and K-Ras4B). H-Ras ΔC (aa 1 - 171) lacks the C-terminus with the CaaX recognition sequence necessary for anchoring Ras into the plasma membrane. The mutation S17N results in a 40-fold increase in the affinity for GTP without affecting its affinity for GDP. Protein preparation is 95% GDP- and 5% GTP-loaded, measured by HPLC.

Referências selecionadas:
Nassar et al. (2010) Structure of the Dominant Negative S17N Mutant of Ras. Biochemistry 49:1970. Sasazuki et al. (2005) Transformation by Oncogenic RAS Sensitizes Human Colon Cells to TRAIL-induced Apoptosis by Up-regulating Death Receptor 4 and Death Receptor 5 through a MEK-dependent Pathway. J Biol. Chem. 280:22856. Wittinghofer et al. (2000) Ras - a molecular switch involved in tumor formation. Angew. Chem. Int. Ed. 39:4192. Feig et al. (1988) Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP. Mol. Cell. Biol. 8:3235.