Non-hydrolyzable GTP Test Kit

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Descrição:
The enzymes involved in hydrolysis of nucleoside triphosphates are a major focus of biochemical research, targeting the understanding and treatment of cellular processes involved in diseases such as cancer, viral infections, cardiovascular and motional disorders. To elucidate the mechanisms of action of these enzymes, it is often desirable to trap the enzymes in their triphosphate-bound state. Since natural nucleotides are hydrolyzed at rates usually preventing such studies, nucleotide analogs are used that are hydrolyzed at much smaller rates or that are even completely resistant to hydrolysis.

The Non-hydrolyzable GTP Test Kit contains a set of 5 typical non-hydrolyzable Guanosine-5'-triphosphate analogs.
The position of the modification within the triphosphate exerts influence on its binding affinity to a particular enzyme and its hydrolysis rate.
This Kit is designed to identify the most suitable non-hydrolyzable GTP derivative for your application.

Contente:
Non-hydrolyzable GuanosineNucleotide || Cat. No. || Amount
GTPαS || NU-409S || 25 μl (100 mM)
GpCpp || NU-405S || 100 μl (10 mM)
GppCp* || NU-402-5 || 5 mg
GppNHp* || NU-401-10 || 10 mg
GTPγS* || NU-412-2 || 2 mg
* Included as a solid for stability reasons. Please dissolve in water and adjust concentration photometrically (extinction coefficient ε252nm = 13.700 M-1cm-1) prior to use to obtain a fresh solution.

Referências selecionadas:
Shiba et al. (2002) Hypocretin stimulates [ (35)]GTP gamma S binding in Hcrtr 2-transfected cell lines and in brain homogenate. Biochem. Bioph. Res. Co. 294 (3):615.

Esters et al. (2001) High-resolution crystal structure of S. cerevisiae Ypt51 (Delta C15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis. J. Mol. Biol. 298 (1):111.

Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.

Muller-Reichert et al. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta) methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95 (7):3661.

Dye et al. (1996) Assembly of microtubules from tubulin bearing the nonhydrolyzable guanosine triphosphate analogue GMPPCP [Guanylyl 5'- (beta,gamma-methylenediphosphonate)]: Variability of growth rates and the hydrolysis of GTP. Biochemistry-US 35 (45):14331.