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  5. Xantosinas e Inosinas – Fluorescência Intrínseca

Xantosinas e Inosinas com Fluorescência Intrínseca

Fluorescent
analog		λabsλemεmaxNotes/Advantages

[nm][nm][l/(mol*cm)]
2',3'-TNP...47055218.500Historically the first fluorescent analogs used as substrates for G-proteins and ATP binding proteins. Also available as pyrimidine probes.
2',3'-Mant...3554485.800"THE CLASSICS" for small GTPases, heterotrimeric G-proteins, GPCRs, and motor protein kinetics. Small MW and environmentally sensitive fluorescence. FRET-probe for protein-intrinsic Tyr and Trp residues.
2',3'-Ant...3324234.600Substitute for Mant-probes. Specific for tubulin, dynein, CaM, and disulfide isomerase. FRET-probe for protein-intrinsic Tyr and Trp residues.
ε-ATP...2754156.000Commonly used substrate of motor proteins, kinases, and other ATP binding proteins.
2-Aminopurine-
riboside-
triphosphate		3203706.000Fluorescent analog of ATP and/or GTP.

Produtos

Mant-XDP NU-210

 

Mant-XTP NU-213

 

Mant-XppNHp NU-211

(Mant-XMPPNP)  

Mant-ITPγS NU-981

 

Selected References

Gromadski et al. (2004) Streptomycin interferes with conformational coupling between codon recognition and GTPase activation on the ribosome. Nature Struct. & Molec. Biol. 11:316.
Gille et al. (2004) Xanthine nucleotide-specific G-protein a-subunits: a novel approach for the analysis of G-protein-mediated signal transduction. NS Arch. Pharmacol. 369:141.
Hiratsuka et al. (2003) You have full text access to this OnlineOpen article. Eur. J. Biochem. 270:3479.
Gille et al. (2003) Fluorescent and colored trinitrophenylated analogs of ATP and GTP. NS Arch. Pharmacol. 368:210.
Bagshaw et al. (2001) ATP analogues at a glance. J. Cell Sci. 114:459.
Jameson et al. (1997) Fluorescent nucleotide analogs: Synthesis and applications. Methods Enzymol. 278:363 and references therein.