EDA-GTP-5/6-TAMRA
2'/3'-O-(2-Aminoethyl-carbamoyl)-Guanosine-5'-triphosphate, labeled with 5/6-TAMRA, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
|---|---|---|---|
| NU-820-TAM | 160 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
excitation and emission spectrum of 5/6-TAMRA
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C38H42N9O19P3 (free acid)
Peso molecular: 1021.72 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λexc 545 nm, λem 575 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Microtubule Gliding Assay [1,2]
Referências selecionadas:
[1] Martin (2013) Measuring microtubule persistence length using a microtubule gliding assay. Methods Cell Biol. 115:13.
[2] Martin et al. (2012) Flexural rigidity measurements of biopolymers using gliding assays. J. Vis. Exp. 69.
Fujimura et al. (2017) Dissection of the angle of single fluorophore attached to the nucleotide in corkscrewing microtubules. Biochem. Biophys. Res. Commun. 485 (3):614.
Lang et al. (2017) Actin ADP-ribosylation at Threonine148 by Photorhabdus luminescens toxin TccC3 induces aggregation of intracellular F-actin. Cell. Microbiol. 19 (1): e12636.
Anderson et al. (2011) A fluorescent GTP analog as a specific, high-precision label of microtubules. BioTechniques 51 (1):43.
Ahmadian et al. (2005) Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins. Biol. Chem. 386:1105.