Immobilized γ-Aminophenyl-m7GTP (C10-spacer)

For general laboratory use.

Envio: shipped at 4 °C

Condições de armazenamento: store at 4 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Formulários:
Suitable for purification of eukaryotic mRNA cap-binding proteins.

Degree of substitution: 5 μmol - 7 μmol m7GTP/ml gel Storage buffer: 20% Ethanol

Referências selecionadas:
Coots et al. (2017) m6A Facilitates eIF4F-Independent mRNA Translation. Mol. Cell. doi:10.1016.

Hou et al. (2017) PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication Viruses 9(9):262.

Theodosakis et al. (2017) p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma by Targeting Protein Synthesis. J. Invest. Dermatol. 137(10):2187.

Arimoto-Matsuzaki et al. (2016) TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat. Commun. 7:10252.

Cinti et al. (2016) HIV-1 Gag Blocks Selenite-Induced Stress Granule Assembly by Altering the mRNA Cap-Binding Complex mBio 7 (2):e00329-16.

Timpano et al. (2016) Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions. J. Vis. Exp. doi:10.3791.

Wahba et al. (2016) Polysome Profiling Links Translational Control to the Radioresponse of Glioblastoma Stem-like Cells. Cancer Res. 76 (10):3078.

Hatakeyama et al. (2014) A Novel Functional Site in the PB2 Subunit of Influenza A Virus Essential for Acetyl-CoA Interaction, RNA Polymerase Activity, and Viral Replication. J. of Biol. Chem. 289:24980.

Igreja et al. (2014) 4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation Nat. Commun. 5:4790.

Morino et al. (1996) Crystallization and preliminary X-ray diffraction study of recombinant human eukaryotic initiation factor-4E. J. Biochem. (Tokyo) 119 (2):224.

Webb et al. (1984) Purification of the messenger RNA cap-binding protein using a new affinity medium. Biochemistry 17,23 (2):177.