DFHO
Mimic of red fluorescent protein (RFP) fluorophore
4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| RNT-003-1 | 1 mg | Sob demanda | Adicionar ao Carrinho |
| RNT-003-5 | 5 x 1 mg | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
store dark
Validade: 12 months after date of delivery
Fórmula molecular: C12H9F2N3O3
Peso molecular: 281.22 g/mol
CAS#: 1420815-34-4
Pureza: ≥ 98 % (HPLC)
Forma: solid
Solubilidade: DMSO
Descrição:
DFHO is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHO-specific RNA sequence (DFHO aptamer) such as Corn.[1-2] DFHO possesses a higher photostability than DFHBI and DFHBI-1T which generally results in a higher signal intensity and lower background in live cell imaging. Fluorescence is activated upon binding to a matching aptamer sequence: Corn/DFHO complex[1]:, Exc.max: 505 nm / Em.max: 545 nm / ɛmax: 29000 M-1 cm-1
Referências selecionadas:
[1] Song et al. (2017) Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat. Chem. Biol. 13(11):1187.
[2] Warner et al. (2017) A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat. Chem. Biol. 13(11):1195.