DFHBI
Mimic of green fluorescent protein (GFP) fluorophore
(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| RNT-001-1 | 1 mg | Sob demanda | Adicionar ao Carrinho |
| RNT-001-5 | 5 x 1 mg | Sob demanda | Adicionar ao Carrinho |
![Structural formula of DFHBI (Mimic of green fluorescent protein (GFP) fluorophore, (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one)](/images/Structures/RNT-001.png)
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
store dark
Validade: 12 months after date of delivery
Fórmula molecular: C12H10F2N2O2
Peso molecular: 252.22 g/mol
CAS#: 1241390-29-3
Pureza: ≥ 98 % (HPLC)
Forma: solid
Solubilidade: DMSO
Descrição:
DFHBI is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHBI-specific RNA sequence (DFHBI aptamer) such as Spinach, Spinach2 or Broccoli.[1-3] Fluorescence is activated upon binding to a matching aptamer sequence: Spinach/DFHBI complex[1]:, Exc.max: 469 nm / Em.max: 501 nm / ɛmax: 24271 M-1 cm-1 Spinach2/DFHBI complex[2]: Exc.max: 447 nm / Em.max: 501 nm / ɛmax: 22000 M-1 cm-1
Referências selecionadas:
[1] Paige et al. (2011) RNA mimics of green fluorescent protein. Science 333(6042):642.
[2] Song et al. (2014) Plug-and-Play fluorophores extend the Spectral Properties of Spinach. J. Am. Chem. Soc. 136:1198.
[3] Filonov et al. (2014) Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution. J. Am. Chem. Soc. 136:16299.