Nucleotídeos Modificados com Amina para Marcação de RNA
Table 1: Enzymatic incorporation of Amine-modified Nucleotides.
cRNA Labeling by in vitro Transcription with... | 3’-RNA Labeling by... | ||||||
---|---|---|---|---|---|---|---|
Nucleotide | Cat. No. | SP6 RNA Polymerase | T7 RNA Polymerase | T3 RNA Polymerase | yPAP | TdT | T4 RNA Ligase |
UTP | |||||||
5-Aminoallyl-UTP | NU-821 | ✔2 | ✔2 | ✔2 | - | n/a | - |
CTP | |||||||
5-Propargylamino-CTP | NU-831 | n/a | n/a | n/a | - | n/a | - |
ATP | |||||||
N6-Aminohexyl-ATP | NU-805 | n/a | n/a | n/a | - | n/a | - |
Cytidine-5'/3'-phosphate | |||||||
pCp-Amine | NU-1706 | - | - | - | - | - | n/a |
TdT: Terminal deoxynucleotidyl Transferase.
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable