LEXSY BHI - Powder Media Kit
brain-heart infusion-based
recommended for transfection and strain maintenance
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| ML-412S | for 1 L | Sob demanda | Adicionar ao Carrinho |
| ML-412L | for 5 L | Sob demanda | Adicionar ao Carrinho |
| ML-412XL | for 10 L | Sob demanda | Adicionar ao Carrinho |
| ML-412XXL | for 50 L | Sob demanda | Adicionar ao Carrinho |
For general laboratory use. Not intended for human or animal diagnostic or therapeutic uses.
Envio: shipped on gel packs
Condições de armazenamento: Store components as indicated on individual labels
Validade: 12 months
Formulários:
For the growth of LEXSY hosts P10 (Cat.-No. LT-101) and T7-TR (Cat.-No. LT-110).
Descrição:
Three-component Kit for preparation of complete Leishmania cultivation medium.
Contente:
LEXSY BHI, powder
Hemin stock solution, 500x (Cat.-No. ML-108):
0,25% solution of Hemin in 30% Triethanolamine, sterile
Pen-Strep stock solution, 200x (Cat.-No. ML-105):
10.000 U/ml of penicillin G sodium salt and 10.000 μg/ml of streptomycin sulfate in 0.85 % saline, sterile
Cat. No. || Content || Amount
ML-412S || 37 g powder2 ml Hemin5 ml Pen-Strep || for 1 l medium
ML-412L || 185 g powder10 ml Hemin25 ml Pen-Strep || for 5 l medium
ML-412XL || 370 g powder20 ml Hemin50 ml Pen-Strep || for 10 l medium
ML-412XXL || 1850 g powder5x 20 ml Hemin250 ml Pen-Strep || for 50 l medium
Storage conditions:
LEXSY BHI, powder:
Store at room temperature
Stable for 12 months
Hemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 month
Pen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month
Preparation of complete Leishmania cultivation medium:
Dissolve 37 g/l LEXSY BHI powder in deionized water and autoclave for 15 min at 121 °C. Control temperature and time with a reference with the same volume, since overexposition leads to de-composition of ingredients and a dark brown color of the medium, which adversely affects growth of LEXSY strains. Proper autoclaved medium is of amber color. Store medium at room temperature before addition of Hemin and Pen-Strep (stable for 12 month). As an alternative, the medium can also be sterile filtered.
Add to 1 l LEXSY BHI:
5.0 ml Pen-Strep solution
2.0 ml Hemin stock solution to a final concentration of 5 μg/ml
Final pH = 7.6 ± 0.2
Store in the dark at 4 °C.
Completed medium is useable for up to 2 weeks. If the completed medium is to be used after this period, appropriate amounts of Hemin and Pen-Strep have to be re-added.
Referências selecionadas:
[1] Zauner et al. (2018) Structural Analyses of Arabidopsis thaliana Legumain γ Reveal Differential Recognition and Processing of Proteolysis and Ligation Substrates. J Biol Chem 293: 8934