LEXSY host P10

For general laboratory use. Not intended for human or animal diagnostic or therapeutic uses.

Envio: Shipped on dry ice

Condições de armazenamento: Store at -80 °C
Avoid freeze/thaw cycles
Upon arrival the glycerol stocks must be stored at -80 °C or inoculated into LEXSY BHI.

Validade: 12 months

Descrição:
The Leishmania tarentolae laboratory strain P10 is used as host strain for the constitutive LEXSY expression vectors.

Contente:
3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host P10 (Leishmania tarentolae laboratory strain P10).

These stocks can be stored at -80 °C for at least 1 year. For reactivation see below.

Organism:
Leishmania tarentolae, laboratory strain P10

Biosafety level:
1, Non-pathogenic for mammalians

Source:
Tarentola annularis

Reactivation of LEXSY host:
Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (Cat.-No. ML-411). Incubate at 26 °C and dilute as required.

Preparation of LEXSY BHI growth medium:
Dissolve 37 g/l LEXSY BHI powder (Cat.-No. ML-412) in deionized water and autoclave for 15 min at 121 °C. Add Hemin and PenStrep. Store at 4 °C and use within two weeks.

Preparation of glycerol stocks:
Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80 % glycerol, mix, incubate 10 min at RT, 1 h on ice and over night at -20 °C. Transfer to -80 °C. Strains can be stored this way for several years.

Referências selecionadas:
[1] Zauner et al. (2018) Structural Analyses of Arabidopsis thaliana Legumain γ Reveal Differential Recognition and Processing of Proteolysis and Ligation Substrates. J Biol Chem 293: 8934