LEXSY: Glicosilação como de Células Mamíferas

Analysis of recombinant human Erythropoietin (EPO) isolated from culture supernatants of a LEXSY expression strain. A: Western blot of commercial recombinant human EPO produced in CHO cells (1) and LEXSY secreted EPO before (2) and following (3) de-glycosylation with N-glycosidase F (PNG). B: Electrophoretic resolution of heterogenous population of CHO-derived EPO. Glycan structures are depicted at left. C: Enzymatic resolution of complex and core glycan structures released from LEXSY-produced EPO (for details refer to Breitling et al. 2002).