SYBR® Green Fluorescent DNA Stain

For in vitro use only!

Envio: Shipped on blue ice

Condições de armazenamento: Store at -20 °C
Store in the dark

Validade: 12 months

Forma: Orange liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20

Concentração: 100 μM

Propriedades espectroscópicas: λexc 495 nm (bound to DNA); λem 520 nm (bound to DNA)

Descrição:
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the
preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument.

SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration.

Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended.

Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay.

Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.

Select the optical setting for SYBR® Green or FAM on the detection instrument.

SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.