SCRIPT Transcriptase Reversa

Transcriptase reversa de alta sensitividade e especificidade

Catálogo Nº Apresentação Preço (R$) Comprar
PRT-102XS 10.000 U371,29 Adicionar ao Carrinho
PRT-102S 20.000 U633,27 Adicionar ao Carrinho
PRT-102M 40.000 U1.076,35 Adicionar ao Carrinho
PRT-102L 100.000 U2.456,80 Adicionar ao Carrinho

For in vitro use only!

Definição de unidade: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37 °C

Envio: Shipped on blue ice

Condições de armazenamento: Store at -20 °C
Avoid freeze/thaw cycles

Validade: 12 months

Pureza: free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease

Forma: Liquid

Concentração: 200 units/μl

Formulários:
Extremely sensitive and highly specific RT-PCR, synthesis of highly structured cDNA fragments, DNA labelling.

Descrição:
SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with reduced Rnase H activity and increased thermal stability. The enzyme is a RNAdirected DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT.

Contente:
SCRIPT 3.0 Reverse Transcriptase (blue cap)
200 units/μl in 20 mM Tris-HCl pH 7.5 (25°C), 5 mM DTT, 50% glycerol (v/v) and stabilizers.
5x SCRIPT 3.0 RT Buffer complete (red cap)
Tris-HCl (pH 8.3), KCl, MgCl2 and DTT.
DTT stock solution (purple cap)
100 mM DTT

Recommended protocols for cDNA synthesis:
Sample denaturation is particularly recommended for RNA targets that exhibit a high degree of secondary structure, for selfor cross complementary primers and for initial experiments
with new targets.
For many standard combinations of RNA and primers heat treatment may be omitted with no negative effect on results.