SCRIPT Transcriptase Reversa
Transcriptase reversa de alta sensitividade e especificidade
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
PRT-102XS | 10.000 U | 371,29 | Adicionar ao Carrinho |
PRT-102S | 20.000 U | 633,27 | Adicionar ao Carrinho |
PRT-102M | 40.000 U | 1.076,35 | Adicionar ao Carrinho |
PRT-102L | 100.000 U | 2.456,80 | Adicionar ao Carrinho |
For in vitro use only!
Definição de unidade: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37 °C
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Avoid freeze/thaw cycles
Validade: 12 months
Pureza: free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease
Forma: Liquid
Concentração: 200 units/μl
Formulários:
Extremely sensitive and highly specific RT-PCR, synthesis of highly structured cDNA fragments, DNA labelling.
Descrição:
SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with reduced Rnase H activity and increased thermal stability. The enzyme is a RNAdirected DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT.
Contente:
SCRIPT 3.0 Reverse Transcriptase (blue cap)
200 units/μl in 20 mM Tris-HCl pH 7.5 (25°C), 5 mM DTT, 50% glycerol (v/v) and stabilizers.
5x SCRIPT 3.0 RT Buffer complete (red cap)
Tris-HCl (pH 8.3), KCl, MgCl2 and DTT.
DTT stock solution (purple cap)
100 mM DTT
Recommended protocols for cDNA synthesis:
Sample denaturation is particularly recommended for RNA targets that exhibit a high degree of secondary structure, for selfor cross complementary primers and for initial experiments
with new targets.
For many standard combinations of RNA and primers heat treatment may be omitted with no negative effect on results.