PC7

For in vitro use only!

Definição de unidade: The activity is defined by the amount of PC7 (μg) that releases 1 pmol AMC from Pyr-Arg-Thr-Lys-Arg-AMC in 1 minute (in 100 mM Hepes/NaOH pH 7.0, 2 mM, 0.2% Triton-X-100 at 37°C with 200 μM Pyr-Arg-Thr-Lys-Arg-AMC).

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 58.8 kDa

Número de acesso: Q16549

Número de acesso: Q16549

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (supplied in 100 mM Hepes/NaOH pH 7.0, 2mM CaCl2, 0.2 % Triton-X-100)

Descrição:
PC7 (proprotein convertase subtilisin/kexin type 7, PCSK7, LPC) is a ubiquitously expressed proprotein convertase involved in the proteolytic maturation of many secreted proteins, such as growth factors, growth factor receptors, serum proteins, coagulation factors and prohormones. PCs also activate proteins of bacterial and viral pathogens and are associated with pathologies like cancer and autoimmune diseases. Recombinant PC7 is expressed in the human HEK293S cell line facilitating homogenous glycosylation of the protein. Ca2+ is crucial for activity; avoid calcium chelators like citrate, EDTA or EGTA in buffers.
For dilution in enzymatic assays, include detergents (e.g. 0.2% Triton-X-100) or BSA (e.g. 1mg/ml) in buffers.

Referências selecionadas:
Dahms et al. (2021) OFF-State-Specific Inhibition of the Proprotein Convertase Furin. ACS Chem. Biol. 10.1021 acschembio.1c00411