Furin

For in vitro use only!

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 52.3 kDa

Número de acesso: P09958

Número de acesso: P09958

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (supplied in 10 mM Hepes/NaOH pH 7.4, 100 mM NaCl, 2mM CaCl2)

Descrição:
Furin (proprotein convertase subtilisin/kexin type 3, PCSK3, PACE) belongs to the proprotein convertases involved in the proteolytic maturation of many proteins in the secretory pathway. In addition to cellular substrates, furin activates the proteins of many bacterial and viral pathogens, including the Spike-protein of the severe acute respiratory virus 2 (SARS-CoV-2). Recombinant human furin is expressed in the human HEK293S cell line facilitating homogenous glycosylation. Active furin is selectively purified using inhibitor based affinity purification. The protein is suited for enzymatic assays, activation of furin’s substrate proteins and protein crystallography. Ca2+ is crucial for activity; avoid calcium chelators like citrate, EDTA or EGTA in buffers. For dilution below 0.1 mg/ml include detergents (e.g. 0.2% Triton-X-100) in buffers. The activity is defined by the amount of furin (μg) that releases 1 pmol AMC from Pyr-Arg-Thr-Lys-Arg-AMC in 1 minute (in 100 mM Hepes/NaOH pH 7.0, 2 mM, 0.2% Triton-X-100 at 37°C with 200 μM Pyr-Arg-Thr-Lys-Arg-AMC).

Referências selecionadas:
Kuester et al. (2011) Purification of the proprotein convertase furin by affinity chromatography based on PC-specific inhibitors. 392(11):973-81
Dahms et al. (2014) X-ray Structures of Human Furin in Complex with Competitive Inhibitors. ACS Chem. Biol. 9(5):1113–1118
Dahms et al. (2016) OFF-State-Specific Inhibition of the Proprotein Convertase Furin. ACS Chem. Biol. 10.1021 acschembio.1c00411