Cdc25A

For general laboratory use.

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 68 kDa

Número de acesso: M81933

Número de acesso: M81933

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 20 mM Tris-HcL pH 8.0, 100 mM NaCl, 1 mM EDTA and 1 mM DTT)

Atividade: 1 nmol of Cdc25A will hydrolyze 10 nmol of pNPP per minute at 37°C at pH 8.0.

Descrição:
The dual specific Cdc25A (Cell division cycle) phosphatase regulates cell cycle progression, has oncogenic and anti-apoptotic activity, and is overexpressed in many human tumors. The protein does not have tags.

Assay buffer: 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 0.1 mM EDTA.

Referências selecionadas:
Fauman et al. (1998) Crystal structure of the catalytic domain of the human cell cycle control phosphatase, Cdc25A. Cell 93:617.

Gottlin et al. (1996) Kinetic analysis of the catalytic domain of human cdc25B. J. Biol. Chem. 271:27445.