HEV-ORF3 (residues 92-123)

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Pureza: > 95 % (SDS-PAGE)

Forma: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 10 mM beta-mercaptoethanol and 8 M urea)

Formulários:
Antigen in ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.

Descrição:
The E. coli derived HEV protein is fused with beta-galactosidase at the N-Terminus. The protein contains immunodominant HEV ORF3 fragment, amino acids: 92-123. It is purified by proprietary chromatographic techniques. Background: Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function.

Specificity: Immunoreactive with sera of HEV-infected individuals.

Referências selecionadas:
Surjit et al. (2004) The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA. J. Virol. 78:320. Tyagi et al. (2001) The full-length and N-terminal deletion of ORF2 protein of hepatitis E virus can dimerize. Biochem. Biophys. Res. Commun. 286:214. Tuteja et al. (2000) Augmentation of immune responses to hepatitis E virus ORF2 DNA vaccination by codelivery of cytokine genes. Viral. Immunol. 13:169. Li et al. (2000) Recombinant subunit ORF2.1 antigen and induction of antibody against immunodominant epitopes in the hepatitis E virus capsid protein. J. Med. Virol. 60:379.