C-HCV

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 70 kDa

Pureza: > 95 % (SDS-PAGE)

Forma: liquid (Supplied in 50 mM NaPO4 pH 8.5, 2.4 mM EDTA, 5 mM DTT and 0.1% SDS)

Formulários:
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Descrição:
The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions. Combined Hepatitis C Virus protein is purified by proprietary chromatographic techniques. Background: Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then constructed into one recombinant antigen.

Specificity: Immunoreactive with sera of HCV-infected individuals.

Referências selecionadas:
Kang et al. (2005) Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. Proteomics 5:2227. Fukutomi et al. (2005) Hepatitis C virus core protein stimulates hepatocyte growth: Correlation with upregulation of wnt-1 expression. Hepatology 41:1096. Gaudy et al. (2005) Usefulness of the hepatitis C virus core antigen assay for screening of a population undergoing routine medical checkup. J. Clin. Microbiol. 43:1722. Lindh et al. (2005) Monitoring treatment response by the hepatitis C virus core antigen assay. Eur. J. Clin. Microbiol. Infect. Dis. 24:230. Boni et al. (2005) Hepatitis C Virus Core Protein Acts as a trans-Modulating Factor on Internal Translation Initiation of the Viral RNA. J. Biol. Chem. 280:17737.