SARS-ACM (residues 182-216)

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 30 kDa

Pureza: > 95 % (SDS-PAGE)

Forma: liquid (Supplied in 50 mM Tris-HCl, 60 mM NaCl and 50% glycerol)

Formulários:
Recombinant SARS-ACM Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.

Descrição:
SARS-ACM contains the matrix protein immunodominant regions, amino acids: 182-216. SARS-ACM is purified by proprietary chromatographic techniques. Background: SARS (Severe Acute Respiratory Syndrome) Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.

Specificity: Immunoreactive with sera of SARSinfected individuals.

Referências selecionadas:
Liu et al. (2004) High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris. World J. Gastroenterol. 10:3602. Luo et al. (2004) Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A. Biochem. Biophys. Res. Commun. 321:557. Wang et al. (2004) Low stability of nucleocapsid protein in SARS virus. Biochemistry 43:11103. Lau et al. (2004) Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay. J. Clin. Microbiol. 42:2884. Woo et al. (2004) Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus. Clin. Diagn. Lab. Immunol. 11:665. Leung et al. (2004) Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid. J. Infect. Dis. 190:379.