SARS-ACE (residues 1-76)

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 34 kDa

Pureza: > 95 % (SDS-PAGE)

Forma: liquid (Supplied in 50 mM Tris-HCl, 60 mM NaCl and 50% glycerol)

Formulários:
Recombinant SARS-ACE Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.

Descrição:
SARS-ACE contains the N-terminus Envelope protein immunodominant regions, amino acids: 1-76. SARS-ACE is purified by proprietary chromatographic techniques.

Background: SARS (Severe Acute Respiratory Syndrome) Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.

Specificity: Immunoreactive with sera of SARS infected individuals.

Referências selecionadas:
Liao et al. (2004) Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability. Biochem. Biophys. Res Commun. 325:374.

Shen et al. (2003) Small envelope protein E of SARS: cloning, expression, purification, CD determination, and bioinformatics analysis. Acta Pharmacol. Sin. 24:505.