Proteinase K - Solid
from Tritirachium album
Endopeptidase K
| Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
|---|---|---|---|
| EN-181-1G | 1 g | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Definição de unidade: One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from haemoglobin as 1 μmol of tyrosine.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles
Validade: 6 months
Peso molecular: 28.9 kDa
CAS#: 39450-01-6
Número CE: 254-457-8
Pureza: free of RNases, DNases and Exonucleases
Forma: powder
Atividade:
Specific activity: > 30 units/mg
DNase activity: not detectable
RNase activity: not detectable
Formulários:
Digestion of proteins during DNA and RNA preparation.
Descrição:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4 - 12.5).
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
In presence of 0.5 - 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.
