Turbonuclease
Encoded by the same gene of Benzonase®
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
ENZ-103S | 5.000 unidades | 282,98 | Adicionar ao Carrinho |
ENZ-103M | 25.000 unidades | 1.085,70 | Adicionar ao Carrinho |
ENZ-103L | 2 x 25.000 unidades | 1.732,50 | Adicionar ao Carrinho |
ENZ-103XL | 500.000 unidades | 13.860,00 | Adicionar ao Carrinho |
For in vitro use only!
Definição de unidade: One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a Δ260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Envio: shipped on blue ice
Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles
Validade: 12 months
Forma: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2, 1 mM DTT and 50 % [v/v] glycerol)
Concentração: 250 units/μl
Formulários:
Turbo Nuclease is very effective in degrading nucleic acid from protein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required:
- Reduction of viscosity from cell lysates
- Prevention of cell clumping
- Elimination of unspecific protein-DNA complexes prior 2D- or native gel electrophoresis
- Removal of nucleic acids from large-scale protein preparations
Descrição:
Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single- or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner.
- Endonuclease from Serratia macescens recombinant expressed and purified from E. coli
- Catalytic activity from pH 6 to 10 (optimal around 8.8) and temperature 0 to 44 °C
- High catalytic efficiency (34-fold greater than DNase I)
- Activity requires the presence of Mg2+ (optimum 2 mM)
Procedure:
- Make a fresh, cold lysis buffer in which the target protein is soluble and is compatible with downstream purification processes, e.g. minimal amount of EDTA or DTT if a Ni-NTA column will be used.
- Resuspend the thawed cell paste in lysis buffer. Use 2-10 ml Lysis Buffer for each gram of cell paste.
- Add Turbo Nuclease to 2.5 units/ml.
- A fluid 'aqueous' solution will result after 15 min.
Referências selecionadas:
Benedik et al. (1998) Serratia marcescens and its extracellular nuclease. FEMS Microbiol. Lett. 165 (1):1.