Shrimp Alkaline Phosphatase - SAP - 1 U/μL
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| ENZ-111XS | 500 U | 372,75 | Adicionar ao Carrinho |
| ENZ-111S | 2 x 500 U | 677,25 | Adicionar ao Carrinho |
| ENZ-111M | 5 x 500 U | 1.692,60 | Adicionar ao Carrinho |
Definição de unidade: One unit of rSAP releases 1 μmol phosphate/min from 4-nitrophenyl phosphate in 0.1 M glycineNaOH pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 and 6 mM 4-nitrophenyl phosphate
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Storage at 4 °C possible for at least 6 months
Storage at 25 °C possible for at least 3 months
Validade: 12 months
Pureza: > 95 % (SDS-PAGE)
Forma: Liquid
Concentração: 1 unit/μL
Atividade:
> 2000 units/mg
Optimum working range is between pH 7-9. rSAP is active in most restriction- and PCR-buffers. Mg2+ (>1 mM) is required for activity.
Descrição:
Recombinant Shrimp Alkaline Phosphatase is a multipurpose alkaline phosphatase that can be fully inactivated by a short heat treatment. This property simplifies most workflows involving
alkaline phosphatase treatment. The recombinant form of SAP replaces the native form that has been established on the market for several years. rSAP has all the properties of the well proven SAP, but with additional benefits. rSAP is far more stable at ambient temperature, it is of high, consistent purity, and is available in large batches at high concentration.
• Heat-labile, all-purpose alkaline phosphatase
• Fast and easy dephosphorylation of DNA, RNA and
nucleotides
• Active in most restriction enzyme buffers, no
need for extra addition of buffer or ions
• Excellent stability at 4°C and even room
temperature
Heat inactivation:
rSAP is completely inactivated by a 5 min incubation at 65 °C.
Absence of contaminants:
Tested for the absence of exonucleases, endonucleases and Rnases.
Produtos relacionados:
- Exonuclease I - #ENZ-100
- EXO + SAP Kit - #DPK-100