ScaI

Isoschizomers: Acc113I, ZrmI

JBSpeed Restriction Enzyme

Catálogo Nº Apresentação Preço (R$) Comprar
EN-131S 1.200 UnitsSob demanda Adicionar ao Carrinho
EN-131L 5 x 1200 UnitsSob demanda Adicionar ao Carrinho
5'–AGTACT–3'
3'–TCATGA–5'

For general laboratory use.

Definição de unidade: One unit is the amount of enzyme required to completely digest 1 μg of Lambda DNA (5 sites) in 1 hour in a total reaction volume of 50 μl. Enzyme activity was determined in the recommended reaction buffer.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Forma: liquid (Supplied in 10 mM Tris-HCl pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50 % [v/v] glycerol)

Concentração: 10 units/μl

Source: Streptomyces caespitosus Supplied with: 10x Universal Buffer (UB) Recommended 50 μl assay5 μl||10x Universal Buffer (UB) 1 μg||pure DNA1 or PCR product2 10 units||enzyme fill up to 50 μl||PCR grade water 1 Supercoiled or high molecular weight DNA (e.g. plant genomic DNA) may require longer incubation time or higher amount of enzyme. 2 Some enzymes may require additional DNA bases flanking the restriction site for complete digestion. Protocol:The enzyme should not exceed 10 % of total reaction volume.Add enzyme as last component. Mix components well before adding enzyme. After enzyme addition, mix gently by pipetting. Do not vortex.Incubate 5 to 10 min. at 37 °C.Stop reaction by alternatively: - Addition of 2.1 μl EDTA pH 8.0 [0.5 M], final 20 mM - Heat Inactivation (20 min. at 80 °C) - Spin Column DNA Purification (e.g. PCR Purification Kit, Cat.-No. PP-201S/L) - Gel Electrophoresis and Single Band Excision (e.g. Agarose Gel Extraction Kit, Cat.-No. PP-202S/L) - Phenol-Chloroform Extraction or Ethanol Precipitation. Double Digestion - Buffer Compatibility: B1 - 90% of the DNA fragments can be ligated and recut with this enzyme. DNA Methylation: No Inhibition: dam, dcm, CpG Quality Control: All preparations are assayed for contaminating endonuclease, 3'-exonuclease, 5' exonuclease/ 5' phosphatase, as well as nonspecific single- and doublestranded DNase activities.

Produtos relacionados: Universal Restriction Buffer, #EN-300