dNTP mix 10 mM

Premix Equimolar de 10 mM dATP, dCTP, dGTP e dTTP

2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt

Catálogo Nº Apresentação Preço (R$) Comprar
NUC-100S 100 μL84,00 Adicionar ao Carrinho
NUC-100M 200 μL126,00 Adicionar ao Carrinho
NUC-100L 1.000 μL420,00 Adicionar ao Carrinho
NUC-100XL 4 x 1.000 μL1.512,00 Adicionar ao Carrinho
Structural formula of dNTP mix 10 mM (Premix Equimolar de 10 mM dATP, dCTP, dGTP e dTTP, 2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt)
Structural formula of dNTP mix 10 mM

For in vitro use only!

Envio: shipped on blue ice

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Validade: 12 months

Fórmula molecular:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)

Peso molecular:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)

Pureza: ≥ 99 % (HPLC)

Forma: clear aqueous solution

pH: 8.5 ±0.2 (22 °C)

Formulários:
For standard PCR applications a final concentration of 200 μM each dNTP is recommended.

Descrição:
dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).

Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable

Referências selecionadas:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.