Bst Polymerase 2.0
Bst polimerase para amplificação isotérmica LAMP
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| POL-133XS | 500 U | 157,50 | Adicionar ao Carrinho |
| POL-133S | 2.000 U | 504,00 | Adicionar ao Carrinho |
| POL-133L | 10.000 U | 2.016,00 | Adicionar ao Carrinho |
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Validade: 12 meses
Concentração: 8 unidades/μL
Descrição:
Bst 2.0 Polymerase is a genetically enhanced Bst polymerase of the next generation. The polymerase is the ideal choice for robust amplification of DNA at constant temperature (60 to 65 °C). The enzyme shows high strand displacement activity and
generates an amplification factor of up to 109 which is comparable to approx. 30 cycles in a PCR assay. The polymerase allows detection of a target gene within 30-60 minutes.
Contente:
Bst 2.0 Polymerase (blue cap)
8 units/μl Bst DNA Polymerase in 10 mM Tris-HCl, 50 mM KCl, 50 % (v/v) Glycerol, pH 7.5 (25 °C) and stabilizers.
Isothermal Buffer (red cap) - 10x conc.
200 mM Tris-HCl pH 8.8, KCl, NH4)2SO4, 20 mM MgSO4 and detergents.
MgSO4 Stock Solution (yellow cap)
25 mM MgSO4.
Detection
Although some methods have been developed to visualize DNA amplification by basic equipment or even the naked eye (increase of turbidity, color change of added dyes, hybridization to gold-bound ss-DNA) in general real-time detection of the DNA amplification by a fluorescent DNA-intercalator dye is recommended. Addition of EvaGreen Fluorescent DNA Stain (#PCK-122, not provided) to the assay allows a sensitive measurement of the increasing amount of DNA without influence on the reaction.