γ-[(6-Azidohexyl)-imido]-ATP

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C16H28N9O12P3 (free acid)

Peso molecular: 631.37 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solid

Solubilidade: 10 mM Tris-HCl pH 7.5

Propriedades espectroscópicas: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
in vitro phosphorylation of recombinant proteins^[1]

Descrição:
Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were γ-[(6-Azidohexyl)-imido]-ATP (compound 6[1]) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2.

The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry.

Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH

Produtos relacionados: γ-[(Propargyl)-imido]-ATP, #CLK-T11, compound 1[1] γ-[2-Azidoethyl]-ATP, #NU-1701, compound 8[1] Copper (II)-Sulphate (CuSO4), #CLK-MI004 Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010 Sodium Ascorbate (Na-Ascorbate), #CLK-MI005

Referências selecionadas:
[1] Lee et al. (2009) Synthesis and reactivity of novel γ-phosphate modified ATP analogues. Bioorg Med Chem Lett. 19:3804.

[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.

[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.