γ-[(PEG3-Amino)-imido]-ATP-Biotin

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C28H48N9O17P3S (free acid)

Peso molecular: 907.72 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH

Referências selecionadas:
Arora et al. (2017) Unexpected biotinylation using ATP-c-Biotin-LC-PEO-amine as a kinase substrate. Biochem. Biophys. Res. Commun. 432:287. Embogama et al. (2017) K-BILDS: A Kinase Substrate Discovery Tool. Chembiochem. 18 (1):136. Ma et al. (2010) Highly sensitive detection of DNA phosphorylation by counting single nanoparticles. Anal. Bioanal. Chem. 397 (6):2279. Seneviranthne et al. (2012) Kinase-Catalyzed Biotinylation. Curr. Protoc. Chem. Biol. 4 (1):83. Seneviranthne et al. (2016) The generality of kinase-catalyzed biotinylation. Biorg. Med. Chem. 24 (1):12. Green et al. (2007) Kinase-catalyzed biotinylation for phosphoprotein detection. J. Am. Chem. Soc. 129 (1):10.