Azide-PEG4-aminoallyl-dUTP
5-(15-Azido-4,7,10,13-tetraoxa-pentadecanoyl-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| NU-1705S | 50 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
| NU-1705L | 5 x 50 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C23H39N6O19P3 (free acid)
Peso molecular: 796.51 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 10 mM - 11 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[1]
Descrição:
Azide-PEG4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice
to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasksto introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for subsequent microscopic imagingto crosslink the DNA to Azide- or DBCO-functionalized biomolecules e.g.proteins
Referências selecionadas:
[1] Sorensen et al. (2013) Enzymatic Ligation of Large Biomolecules to DNA. ACS Nano 7 (9):8098.