AppCp

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C11H18N5O12P3 (free acid)

Peso molecular: 505.21 g/mol (free acid)

CAS#: 7414-56-4

Pureza: ≥ 95 % (HPLC)

Forma: solid

Propriedades espectroscópicas: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Conformational studies on complex with mevalonate diphosphate decarboxylase^[1]
Structure of the complex with folylpolyglutamate synthetase^[2]
Structure of complex with p90 ribosomal S6 kinases^[3]
Crystal structure of complex with Ca2+-ATPase^[4]
NMR-studies of complex with yeast hexokinase^[5]
Agonistic ligand, mainly for nucleoside receptor A₁
Nucleosidephosphates stabilized against hydrolytic degradation can directly bind to nucleoside receptors.

Specific Ligands:

Tubulin[6]

Nonhydrolyzable ligand for P2X purinoreceptor[7,8]

Referências selecionadas:
[1] Reuther et al. (2011) Backbone 1H, 13C, 15N NMR assignments of the unliganded and substrate ternary complex forms of mevalonate diphosphate decarboxylase from Streptococcus pneumoniae. Biomol. NMR Assign. 5 (1):11.

[2] Young et al. (2008) Structures of Mycobacterium tuberculosis folylpolyglutamate synthase complexed with ADP and AMPPCP. Acta Crystallogr D Biol Crystallogr. D64 (Pt 7):745.

[3] Ikuta et al. (2007) Crystal structures of the N-terminal kinase domain of human RSK1 bound to three different ligands: Implications for the design of RSK1 specific inhibitors. Protein Sci. 16 (12):2626.

[4] Picard et al. (2005) The average conformation at micromolar [Ca2+] of Ca2+-atpase with bound nucleotide differs from that adopted with the transition state analog ADP.AlFx or with AMPPCP under crystallization conditions at millimolar [Ca2+]. J. Biol. Chem. 280 (19):18745.

[5] Maity et al. (2002) Structure of triphosphoryl nucleotide bound at the active site of yeast hexokinase: 1H-nuclear magnetic resonance study. J. Protein Chem. 21 (4):265.

[6] Kubala (2006) ATP-binding to P-type ATPases as revealed by biochemical, spectroscopic, and crystallographic experiments. Proteins. 64 (1):1.

[7] Trezise et al. (1995) The selective P2X purinoceptor agonist, beta,gamma-methylene-L-adenosine 5'-triphosphate, discriminates between smooth muscle and neuronal P2X purinoceptors. Arch. Pharmacol. 351 (6):603.

O'Connor et al. (1990) Characterization of P2x-receptors in rabbit isolated ear artery. Br. J. Pharmacol. 101 (3):640.

Del Toro Duany et al. (2008) The reverse gyrase helicase-like domain is a nucleotide-dependent switch that is attenuated by the topoisomerase domain. Nucleic Acids Research 36 (18):5882.

Toyoshima et al. (2004) Crystal structure of the calcium pump with a bound ATP analogue. Nature 430:529.

Mayer et al. (1991) Re-evaluation of the specificity of adenylyl (beta,gamma-methylene)diphosphonate as a substrate for adenylate cyclase. Histochem J. 23 (2):100.

Williams et al. (1986) Effects of purine nucleotides on the binding of [3H]cyclopentyladenosine to adenosine A1-receptors in rat brain membranes. J. Neurochem. 47 (1):88.

Mayer et al. (1985) Specificity of cytochemical demonstration of adenylate cyclase in liver using adenylate- (beta, gammamethylene) diphosphate as substrate. Histochemistry. 82 (2):135.

Meghji et al. (1984) The effect of adenylyl compounds on the heart of the dogfish, Scyliorhinus canicula. Comparative Biochem. and Physiol.Part C: Pharmacol., Toxicol.& Endocrin. 77C (2):295.

Meghji et al. (1983) The effect of adenylyl compounds on the heart of the axolotl (Ambystema mexicanum). Comparative Biochem. and Physiol.Part C: Pharmacol., Toxicol.& Endocrin. 76 (2):319.