0 Itens
  1. You are here:
  2. Nucleotídeos e Nucleosídeos
  3. Nucleotídeos por Estrutura
  4. Nucleotídeos Não Hidrolisáveis
  5. Nucleotídeos Não Hidrolisáveis α,β
  6. Nucleotídeos Guanosina
  7. GpCp

GpCp

(GMPCP)

Guanosine-5'-[(α,β)-methyleno]diphosphate, Sodium salt

Datasheet (PDF)
Catálogo Nº Apresentação Preço (R$) Comprar
NU-414-5 5 mgSob demanda Adicionar ao Carrinho
NU-414-25 25 mgSob demanda Adicionar ao Carrinho
Structural formula of GpCp ((GMPCP), Guanosine-5'-[(α,β)-methyleno]diphosphate, Sodium salt)
Structural formula of GpCp

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C11H17N5O10P2 (free acid)

Peso molecular: 441.23 g/mol (free acid)

CAS#: 32381-15-0

Pureza: ≥ 95 % (HPLC)

Forma: solid

Propriedades espectroscópicas: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Hydrolysis by tubulin[1]
Influence on Z-DNA conformation[2]
1H-NMR-spectra[3]

Referências selecionadas:
[1] Caplow et al. (1994) The free energy of hydrolysis of a microtubule-bound nucleotide triphosphate is near zero: All of free energy for hydrolysis is stored in microtubule lattice. J. Cell Biology 127:779.

[2] Ban et al. (1996) Crystal structure of the self-complementary 5'-purine start decamer d (GCGCGCGCGC) in the Z-DNA conformation. Biophysical J. 71:1215.

[3] Inoue et al. (1969) Low-field proton magnetic resonance spectra for seven dinucleotides ApCp, ApGp, ApUp, CpGp, GpCp, GpUp, and UpGp. Physiological Chemistry and Physics 1:77.

Martin-Galiano et al. (2011) Bacterial tubulin distinct loop sequences and primitive assembly properties support its origin from a eukaryotic tubulin ancestor.J. Biol. Chem. 286 (22):19789.

Muller-Reichert et al. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta)methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95 (7):3661.

Vulevic et al. (1997) Role of guanine nucleotides in the vinblastine-induced self-association of tubulin: effects of guanosine alpha,betamethylenetriphosphate and guanosine alpha,beta-methylenediphosphate. Biochemistry-US 36 (42):12828.

Hyman et al. (1995) Structural-changes accompanying GTP hydrolysis in microtubules - information from a slowly hydrolyzable analog guanylyl- (alpha,beta)-methylene-diphosphonate. J. Cell Biol. 128 (1-2):117.

Hyman et al. (1992) Role of GTP hydrolysis in microtubule dynamics - information from a slowly hydrolyzable analog, GMPCPP. Mol. Biol. Cell 3 (10):1155.