dGTPαS
2'-Deoxyguanosine-5'-(α-thio)-triphosphate, Sodium salt; (Mixture of Rp and Sp isomers)
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| NU-424S | 100 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
| NU-424L | 5 x 100 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C10H16N5O12P3S (free acid)
Peso molecular: 523.24 g/mol (free acid)
CAS#: 82337-56-2
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 10 mM - 11 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Referências selecionadas:
Gaur et al. (1993) Enzymatic RNA synthesis with deoxynucleoside 5'-O- (1-thiotriphosphates). FEBS Lett. 315 (1):56.
Abbotts et al. (1988) Studies on the mechanism of Escherichia coli DNA polymerase I large fragment. Effect of template sequence and substrate variation on termination of synthesis. J. Biol. Chem. 263 (29):15094.
Nakamaye et al. (1988) Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates. Nucleic Acids Res. 16 (21):9947.
Nyren et al. (1997) Detection of single-base changes using a bioluminometric primer extension assay. Anal. Biochem. 244 (2):367.