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GTPαS

Guanosine-5'-(α-thio)-triphosphate, Sodium salt; (Mixture of Rp and Sp isomers)

Datasheet (PDF)
Catálogo Nº Apresentação Preço (R$) Comprar
NU-409S 25 μl (100 mM)Sob demanda Adicionar ao Carrinho
NU-409L 5 x 25 μl (100 mM)Sob demanda Adicionar ao Carrinho
Structural formula of GTPαS (Guanosine-5'-(α-thio)-triphosphate, Sodium salt; (Mixture of Rp and Sp isomers))
Structural formula of GTPαS

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C10H16N5O13P3S (free acid)

Peso molecular: 539.24 g/mol (free acid)

CAS#: 81570-51-6

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 100 mM - 110 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Stability against decapping scavenger pyrophosphatase[1]
Inhibition of guanylate cyclase[2]

Specific Ligands:

Translational factor eIF4E[1]

Bacterial diguanylate cyclase[3]

Referências selecionadas:
[1] Kowalska et al. (2009) Phosphorothioate analogs of m7GTP are enzymatically stable inhibitors of cap-dependent translation. Bioorganic and Medicinal Chemistry Letters 19:1921.

[2] Garger et al. (2001) Inhibitors of guanylate cyclase inhibit phototransduction in limulus ventral photoreceptors. Visual Neuroscience 18:625.

[3] Wassmann et al. (2007) Structure of BeF3-modified response regulator PleD: implications for diguanylate cyclase activation, catalysis and feedback inhibition. Structure (London) 15:915.

Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.

Strobel (1999) A chemogenetic approach to RNA function/structure analysis. Curr. Opin. Struc. Biol. 9 (3):346.

Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.

Antonny et al. (1993) GTP hydrolysis by purified alpha-subunit of transducin and its complex with the cyclic-GMP phosphodiesterase inhibitor. Biochemistry-US 32 (33):8646.