EDA-ATP
2'/3'-O-(2-Aminoethyl-carbamoyl)-Adenosine-5'-triphosphate, Sodium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| NU-808S | 30 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
| NU-808L | 5 x 30 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C13H22N7O14P3 (free acid)
Peso molecular: 593.27 g/mol (free acid)
CAS#: 173074-70-9, 152270-20-7
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 10 mM - 11 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Coupling of Cy3 and Cy5[1]
Referências selecionadas:
[1] Oiwa et al. (2000) Comparative Single-Molecule and Ensemble Myosin Enzymology: Sulfoindocyanine ATP and ADP Derivatives. Biophys. J. 78:3048.
Komori et al. (2008) Measurement system for simultaneous observation of myosin V chemical and mechanical events. BioSystems 93:48.
Rumjahn et al. (2007) Purinergic regulation of angiogenesis by human breast carcinoma-secreted nucleoside diphosphate kinase. British Journal of Cancer 97:1372.
Jameson et al. (1997) Fluorescent analogs: Synthesis and Applications. Methods in Enzymology 278:363.
Eccleston et al. (1996) Ribose-linked sulfoindocyanine conjugates of ATP: Cy3-EDA-ATP and Cy5-EDA-ATP. Biophys. J. 70 (2): MPO29 Part 2.
Oiwa et al. (1996) Microscopic observations of Cy3-EDA-ATP and Cy5-EDA-ATP binding to myosin filaments in vitro. Biophys. J. 70 (2):MPO30 Part 2.
Conibear et al. (1996) Measurement of nucleotide exchange kinetics with isolated synthetic myosin filaments using flash photolysis. FEBS Lett. 380 (1-2):13.
Conibear et al. (1996) Kinetic and spectroscopic characterization of fluorescent ribose-modified ATP analogs upon interaction with skeletal muscle Myosin subfragment 1. Biochemistry 35 (7):2299.
Watson et al. (1995) Macromolecular arrangement in the aminoacyl-transfer-RNA-elongation factor-gtp ternary complex - a fluorescence energy-transfer study. Biochemistry 34 (24):7904.
Braxton et al. (1988) The synthesis of a novel class of ribose-modified nucleotide analogs. 1. affinity purification of skeletal Myosin subfragment-1. Biophys. J. 53 (2):A178 Part 2.