EDA-ADP

2'/3'-O-(2-Aminoethyl-carbamoyl)-Adenosine-5'-diphosphate, Sodium salt

Catálogo Nº Apresentação Preço (R$) Comprar
NU-802S 30 μl (10 mM)Sob demanda Adicionar ao Carrinho
NU-802L 5 x 30 μl (10 mM)Sob demanda Adicionar ao Carrinho
Structural formula of EDA-ADP (2'/3'-O-(2-Aminoethyl-carbamoyl)-Adenosine-5'-diphosphate, Sodium salt)
Structural formula of EDA-ADP

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C13H21N7O11P2 (free acid)

Peso molecular: 513.30 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 10 mM - 11 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Referências selecionadas:
Oiwa et al. (2000) Comparative Single-Molecule and Ensemble Myosin Enzymology: Sulfoindocyanine ATP and ADP Derivatives. Biophys. J. 78:3048.

Jameson et al. (1997) Fluorescent analogs: Synthesis and Applications. Methods in Enzymology 278:363.

Oiwa et al. (1997) Microscopic kinetic measurements of single Cy3-EDA-ADP molecules interacting with Myosin filaments in vitro. Biophys. J. 72 (2):TU240 Part 2.

Eccleston et al. (1996) Ribose-linked sulfoindocyanine conjugates of ATP: Cy3-EDA-ATP and Cy5-EDA-ATP. Biophys. J. 70 (2): MPO29 Part 2.

Oiwa et al. (1996) Microscopic observations of Cy3-EDA-ATP and Cy5-EDA-ATP binding to myosin filaments in vitro. Biophys. J. 70 (2):MPO30 Part 2.

Conibear et al. (1996) Measurement of nucleotide exchange kinetics with isolated synthetic myosin filaments using flash photolysis. FEBS Lett. 380 (1-2):13.

Conibear et al. (1996) Kinetic and spectroscopic characterization of fluorescent ribose-modified ATP analogs upon interaction with skeletal muscle Myosin subfragment 1. Biochemistry 35 (7):2299.

Watson et al. (1995) Macromolecular arrangement in the aminoacyl-transfer-RNA-elongation factor-gtp ternary complex - a fluorescence energy-transfer study. Biochemistry 34 (24):7904.

Braxton et al. (1988) The synthesis of a novel class of ribose-modified nucleotide analogs. 1. affinity purification of skeletal Myosin subfragment-1. Biophys. J. 53 (2):A178 Part 2.