5-DBCO-PEG4-dUTP
5-Dibenzylcyclooctyl-PEG4-uridine-5'-triphosphate, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| CLK-048-S | 50 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
| CLK-048-L | 5 x 50 μl (10 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C42H54N5O21P3 (free acid)
Peso molecular: 1057.83 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in 100 mM Tris-HCl
Concentração: 10 mM - 11 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
Descrição:
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice
to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasksto introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Azide-functionalized biomolecules e.g.proteins