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  7. Biotin-11-dCTP

Biotin-11-dCTP

Biotin-X-5-Propargylamino-dCTP

γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt

Datasheet (PDF)
Catálogo Nº Apresentação Preço (R$) Comprar
NU-809-BIOX-S 200 μl (1 mM)Sob demanda Adicionar ao Carrinho
NU-809-BIOX-L 5 x 200 μl (1 mM)Sob demanda Adicionar ao Carrinho
Structural formula of Biotin-11-dCTP (Biotin-X-5-Propargylamino-dCTP, γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt)
Structural formula of Biotin-11-dCTP

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C28H44N7O16P3S (free acid)

Peso molecular: 859.67 g/mol (free acid)

CAS#: 136632-30-9

Pureza: ≥ 95 % (HPLC)

Forma: filtered solution (30 kDa) in 10 mM Tris-HCl

Concentração: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
FISH[1, 2]
SNP-analysis[1]
Nick-translation[2]
TUNEL[3]

Descrição:
Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.

Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP

Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.

Produtos relacionados:

  • Biotin-16-dUTP, #NU-803-BIO16
  • Biotin-16-dCTP, #NU-809-BIO16
  • Biotin-11-dUTP, #NU-803-BIOX
  • Digoxigenin-11-dUTP, #NU-803-DIGX

Referências selecionadas:
[1] Ye et al. (2001) Fluorescent microsphere-based readout technology for multiplexed human single nucleotide polymorphism analysis and bacterial identification. Human Mutation 17:305.

[2] Backx et al. (2008) Direct fluorescent labelling of clones by DOP PCR. Molecular Cytogenetics 1:3.

[3] Jones et al. (2003) Herpes simplex virus type 2 induces rapid cell death and functional impairment of murine dendritic cells in vitro. J. Virology 77:11139.

Bishop et al. (2008) APOBEC3G Inhibits Elongation of HIV-1 Reverse Transcripts. PLoS Pathogens 4:e1000231.