pCp-Biotin
Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Biotin, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
|---|---|---|---|
| NU-1706-BIO | 20 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C25H42N6O13P2S1 (free acid)
Peso molecular: 728.65 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in 10 mM Tris-HCl
Concentração: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into RNA/miRNA by
- 3'-End Labeling with T4 RNA Ligase
Descrição:
pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.
Produtos relacionados: pCp-Desthiobiotin, #NU-1706-Desthiobio pCp-Cy3, #NU-1706-CY3 pCp-Cy5, #NU-1706-CY5 pCp-Amine, #NU-1706 pCp-Azide, #NU-1708 pCp-Alkyne, #NU-1709
Referências selecionadas:
Kashida et al. (2018) Nanoparticle-based local translation reveals mRNA as translation-coupled scaffold with anchoring function. doi:10.1101/483727.
Booy et al. (2016) RNA Helicase Associated with AU-rich Element (RHAU/DHX36) Interacts with the 3′-Tail of the Long Non-coding RNA BC200 (BCYRN1). J. Biol. Chem. 291 (10):5355.