4-Thio-UDP

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C9H14N2O11P2S (free acid)

Peso molecular: 420.22 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 10 mM - 11 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 331 nm, ε 16.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Referências selecionadas:
Zaher et al. (2006) A general RNA-capping ribozyme retains stereochemistry during cap exchange. J. Am. Chem. Soc. 128 (42):13894.

Kwon et al. (2001) DNA sequencing and genotyping by transcriptional synthesis of chain-terminated RNA ladders and MALDI-TOF mass spectrometry. Nucleic Acids Res. 29 (3):e11.

Testa et al. (1999) Thermodynamics of RNA-RNA Duplexes with 2- or 4-Thiouridines: Implications for Antisense Design and Targeting a Group I Intron. Biochemistry 38:16655.

Dontsova et al. (1994) Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center. Proc. Natl. Acad. Sci. USA 91 (10):4125.

Sheng et al. (1993) Active site labeling of HIV-1 reverse transcriptase. Biochemistry 32 (18):4938.

Khanna et al. (1991) Photoaffinity labelling of the pea chloroplast transcriptional complex by nascent RNA in vitro. Nucleic Acids Res. 19 (18):4849.

Dissinger et al. (1990) Active site labeling of Escherichia coli transcription elongation complexes with 5-((4-azidophenacyl)thio)uridine 5'-triphosphate. J. Biol. Chem. 265 (13):7662.

Tanner et al. (1988) Binding interactions between yeast tRNA ligase and a precursor transfer ribonucleic acid containing two photoreactive uridine analogues. Biochemistry 27 (24):8852.

Bartholomew et al. (1986) RNA contacts subunits IIo and IIc in HeLa RNA polymerase II transcription complexes. J. Biol. Chem. 261 (30):14226.

Eshaghpour et al. (1979) Specific chemical labeling of DNA fragments. Nucleic Acids Res. 7 (6):1485.