m7GTP - Solution

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C11H18N5O14P3 (free acid)

Peso molecular: 537.21 g/mol (free acid)

CAS#: 26554-26-7 (free acid), 104809-18-9 (sodium salt)

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 10 mM - 11 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 258/280 nm, ε 9.8/8.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
X-ray of complex with eIF4ES^{[1, 2]}
Thermodynamic binding analysis to eIF4E^[3]
Affinity chromatogrphy withm7GTP-sepharose^{[4, 5]}

Referências selecionadas:
[1] Ashby et al. (2011) Structure-based mutational analysis of eIF4E in relation to sbm1 resistance to Pea seed-borne mosaic virus in pea. PLoS One 6:e15873.

[2] Brown et al. (2009) Crystallization of eIF4E complexed with eIF4GI peptide and glycerol reveals distinct structural differences around the cap-binding site. Cell Cycle 8:1905.

[3] Guimaraes et al. (2009) Thermodynamic analysis of mRNA cap-binding by the human initiation factor eIF4E via free enrgy perturbations. J. Amer. Chem. Soc. 131:18139.

[4] Yoffe et al. (2009) Evolutionary changes in the Leishmania eIF4F complex involve variations in the eIF4G interactions. Nucleic Acid Research 37:3243.

[5] Szczepaniak et al. (2008) Bisphosphonate mRNA cap analog attached to sepharose for affinity chromatography of decapping enzymes. Nucleic Acids Symposium Series 52:295.

Shen et al. (2001) Structural and thermodynamic behavior of eukaryotic initiation factor 4E in supramolecular formation with 4E-binding protein 1 and mRNA cap analogue, studied by spectroscopic methods. Chem Pharm Bull 49 (10):1299.

Carberry et al. (1989) A spectroscopic study of the binding of m7GTP and m7GpppG to human protein synthesis initiation factor 4E. Biochemistry 28 (20):8078.

Beemon et al. (1977) In vitro translation yields a possible Rous sarcoma virus src gene product. Proc Natl Acad Sci U S A. 74 (8):3302.