m32.2.7GP3G (Trimethylated Cap Analog)

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C23H33N10O18P3 (free acid)

Peso molecular: 830.49 g/mol (free acid)

CAS#: 123212-20-4 (acid)

Pureza: ≥ 95 % (HPLC)

Forma: solid

Propriedades espectroscópicas: λmax 257 nm, λ 260 nm, ε 21.6 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Referências selecionadas:
Roithová et al. (2018) The Sm-core mediates the retention of partially-assembled spliceosomal snRNPs in Cajal bodies until their full maturation. Nucleic Acids Res. 46 (7):3774.

Stepanov et al. (2018) Nucleotide Modifications Decrease Innate Immune Response Induced by Synthetic Analogs of snRNAs and snoRNAs. Genes (Basel) doi: 10.3390.

Abdelhamid et al. (2014) Multiplicity of 5' cap structures present on short RNAs. PLoS One 9 (7):e102895.

Issur et al. (2013) Enzymatic Synthesis of RNAs Capped with Nucleotide Analogues Reveals the Molecular Basis for Substrate Selectivity of RNA Capping Enzyme: Impacts on RNA Metabolism. PLoS One 8 (9):e75310.

Maroney et al. (1995) Most mRNAs in the nematode Ascaris lumbricoides are trans-spliced: a role for spliced leader addition in translational efficiency. RNA 1 (7):714.

Fischer et al. (1991) Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport. J. Cell Biology 113 (4):705.

Hamm et al. (1991) The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal. Cell 62 (3):569.