5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU)

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C31H28N4O7 (free acid)

Peso molecular: 568.58 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solid

Propriedades espectroscópicas: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
DNA synthesis monitoring (potentially)

Descrição:
5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells.
5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine.

The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice tointroduce a Biotin group for subsequent purification tasks (via Azides of Biotin)introduce a fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes).

Produtos relacionados: 5-Ethynyl-2'-deoxy-uridine (5-EdU), #CLK-N001 5-Vinyl-2'-deoxyuridine (5-VdU), #CLK-050 5-Azidomethyl-2'-deoxyuridine(5-AmdU), #CLK-050