Ac4ManNAz
N-azidoacetylatedmannosamine-tetraacylated (Ac4ManAz)
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| CLK-1084-5 | 5 mg | Sob demanda | Adicionar ao Carrinho |
| CLK-1084-25 | 5 x 5 mg | Sob demanda | Adicionar ao Carrinho |
| CLK-1084-100 | 100 mg | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped at ambient temperature
Condições de armazenamento: store at -20 °C
Validade: 12 months after date of delivery
Fórmula molecular: C16H22N4O10
Peso molecular: 430.37 g/mol
CAS#: 361154-30-5
Pureza: mass identification (ESI-MS)
Forma: oil
Solubilidade: DMSO, DMF, MeOH
Formulários:
Glycoconjugate synthesis monitoring by metabolic labeling
Descrição:
The tetraacetylated N-Azidoacetyl-mannosamine (Ac4ManNAz) provides a non-radioactive alternative for glycoconjugate visualization. It is cell-permeable, intracellularly processed and incorporated instead of its natural monosaccharide counterpart N-Acetylmannosamine(ManNAc).
The resulting Azide-functionalized glycoconjugates can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging.
Recommended concentration for metabolic labeling: 25-75 μM. This concentration range may serve as a starting point for an individual assay set-up.
Referências selecionadas:
Spiciarich et al. (2017) Bioorthogonal Labeling of Human Prostate Cancer Tissue Slice Cultures for Glycoproteomis. Angew. Chem. Int. Ed.129:1.