Azide-PEG3-FLAG

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C59H88N16O27S

Peso molecular: 1485.49 g/mol

Pureza: ≥ 95 % (HPLC)

Forma: solid

Solubilidade: water, PBS (up to 30 mM tested)

Descrição:
Azide-PEG3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne- or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively.

The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.

Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 μM)[1]. This concentrations may serve as a starting point for individual assay set-up.

Produtos relacionados: DBCO-PEG4-FLAG, #CLK-033

Referências selecionadas:
[1] Wang et al. (2011) Sulfated Ligands for the Copper (I)-catalyzed Azide-Alkyne Cycloaddition. Chem Asian J. 6 (10):2796.