UDP-6-azide-glucose

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C15H23N5O16P2

Peso molecular: 591.32 g/mol

Pureza: ≥ 98 % (HPLC)

Forma: solid

Solubilidade: water, aqueous buffer such as PBS

Formulários:
Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 ß-Glycosyltransferase (T4 ß-GT)^[1-3]
The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu(I)-free click chemistry that offers the choice

• to introduce a (Desthio)Biotin group for subsequent purification tasks (via DBCO-containing (Desthio)Biotin)
• to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes)

0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 μl of a 3 mM solution.

Referências selecionadas:
[1] Song et al. (2011) Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nature Biotech 29(1):68.
[2] Li et al. (2012) Selective Capture of 5-hydroxymethylcytosine from Genomic DNA. J. Vis. Exp. 68:e4441.
[3] Song et al. (2016) Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation. PNAS 113(16):4339.